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il 18 dy318 05  (R&D Systems)


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    Structured Review

    R&D Systems il 18 dy318 05
    Il 18 Dy318 05, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 214 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+total+il/Human+Total+IL-18+DuoSet+ELISA/pm41991796-83-37-33
    Average 96 stars, based on 214 article reviews
    il 18 dy318 05 - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Concentration Assay:

    Article Title: Evaluating Single-Nucleotide Polymorphisms in Inflammasome Proteins and Serum Levels of IL-18 and IL-1β in Kidney Interstitial Damage in Anti-Neutrophilic Cytoplasmic Antibody-Associated Vasculitis
    Article Snippet: Genotyping was performed using the TaqMan SNP genotyping assay (Applied Biosystems, Foster City, CA, USA) on the 7900HT Fast Real-time PCR System (Thermo Fisher Scientific, Waltham, MA, USA), following standard recommendations. .. The concentration of IL-18 and IL-1β in serum and IL-1β in urine samples were measured using a commercial enzyme-linked immunosorbent assay (ELISA) kit according to the manufacturer’s instructions (Human Total IL-18/IL-1F4 Quantikine ELISA Kit, R&D systems ® , Minneapolis, MN, USA, and IL-1β Human ELISA Kit, High Sensitivity, ThermoFisher ® , Schwerte, Germany, respectively). ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Evaluating Single-Nucleotide Polymorphisms in Inflammasome Proteins and Serum Levels of IL-18 and IL-1β in Kidney Interstitial Damage in Anti-Neutrophilic Cytoplasmic Antibody-Associated Vasculitis
    Article Snippet: Genotyping was performed using the TaqMan SNP genotyping assay (Applied Biosystems, Foster City, CA, USA) on the 7900HT Fast Real-time PCR System (Thermo Fisher Scientific, Waltham, MA, USA), following standard recommendations. .. The concentration of IL-18 and IL-1β in serum and IL-1β in urine samples were measured using a commercial enzyme-linked immunosorbent assay (ELISA) kit according to the manufacturer’s instructions (Human Total IL-18/IL-1F4 Quantikine ELISA Kit, R&D systems ® , Minneapolis, MN, USA, and IL-1β Human ELISA Kit, High Sensitivity, ThermoFisher ® , Schwerte, Germany, respectively). ..

    Article Title: Parquetina nigrescens leaf extract mitigates aflatoxin B1-induced stress, immune and growth disruptions in broilers.
    Article Snippet: Aflatoxin B1 (AFB1) contamination in poultry feed impairs immunity, induces oxidative stress, and reduces growth and carcass quality.. This study assessed the protective role of Parquetina nigrescens leaf extract (PNLE) against AFB1-induced immuno-inflammatory, oxidative, and metabolic disturbances in broiler chickens.. Two hundred day-old Cobb 500 broiler chicks were assigned to four groups: NOC (control), AFCP0 (AFB1 only, 0.5 mg/kg feed), AFCP1 (AFB1 + 1 g PNLE/L water), and AFCP2 (AFB1 + 2 g PNLE/L).

    Article Title: Aging and inflammation limit the induction of SARS-CoV-2–specific CD8 + T cell responses in severe COVID-19
    Article Snippet: Data were acquired using an LSR Fortessa (BD Biosciences) or a FACSymphony A5 (BD Biosciences) and analyzed using FlowJo version 10.8.1 (FlowJo LLC). .. Plasma concentrations of IL-18 were quantified using a Human Total IL-18/IL-1F4 Quantikine ELISA (R&D Systems), and plasma concentrations of IL-18BP were quantified using a Human IL-18 BPa Quantikine ELISA (R&D Systems). ..

    Article Title: Ageing and inflammation limit the induction of SARS-CoV-2-specific CD8+ T cell responses in severe COVID-19.
    Article Snippet: .. Plasma concentrations of IL-18 were quantified using a Human Total IL-18/IL-1F4 Quantikine ELISA (R&D Systems), and plasma concentrations of IL-18BP were quantified using a Human IL-18 BPa Quantikine ELISA (R&D Systems). ..

    Article Title: Impact of HHIP gene polymorphisms on phenotypes, serum IL-17 and IL-18 in COPD patients of the Chinese Han population
    Article Snippet: .. Serum IL-17 levels were analyzed using The Human IL-17 Quantikine HS ELISA Kit HS170 from R&D Systems; Serum IL-18 levels were analyzed using Human Total IL-18/IL-1F4 Quantikine ELISA Kit from R&D Systems. ..

    Article Title: Impact of HHIP gene polymorphisms on phenotypes, serum IL-17 and IL-18 in COPD patients of the Chinese Han population.
    Article Snippet: .. Serum IL-17 levels were analyzed using The Human IL-17 Quantikine HS ELISA Kit HS170 from R&D Systems; Serum IL-18 levels were analyzed using Human Total IL-18/IL-1F4 Quantikine ELISA Kit from R&D Systems. ..

    Article Title: The tetrapeptide sequence of IL-18 and IL-1β regulates their recruitment and activation by inflammatory caspases
    Article Snippet: .. Human Total IL-18/IL-1F4 Quantikine ELISA Kit (R&D Systems, DL180), Mouse IL-1 beta/IL-1F2 Quantikine ELISA Kit (R&D Systems, MLB00C). .. HEK 293T cells, HeLa cells, and THP1 cells were purchased from ATCC.

    Clinical Proteomics:

    Article Title: Aging and inflammation limit the induction of SARS-CoV-2–specific CD8 + T cell responses in severe COVID-19
    Article Snippet: Data were acquired using an LSR Fortessa (BD Biosciences) or a FACSymphony A5 (BD Biosciences) and analyzed using FlowJo version 10.8.1 (FlowJo LLC). .. Plasma concentrations of IL-18 were quantified using a Human Total IL-18/IL-1F4 Quantikine ELISA (R&D Systems), and plasma concentrations of IL-18BP were quantified using a Human IL-18 BPa Quantikine ELISA (R&D Systems). ..

    Article Title: Ageing and inflammation limit the induction of SARS-CoV-2-specific CD8+ T cell responses in severe COVID-19.
    Article Snippet: .. Plasma concentrations of IL-18 were quantified using a Human Total IL-18/IL-1F4 Quantikine ELISA (R&D Systems), and plasma concentrations of IL-18BP were quantified using a Human IL-18 BPa Quantikine ELISA (R&D Systems). ..

    other:

    Article Title: HIT immune complexes activate the inflammasome pathway in a complement-dependent manner.
    Article Snippet: PowerSYBR Green, Trizol reagent, and SuperScript III First-Strand Synthesis System were from Thermo Fisher Scientific (Waltham, MA).



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    Cytokine secretion profile of CALU-3/dTHP-1 coculture following exposure to nebulized LPS. ( A ) IL-6, ( B ) IL-8, ( C ) IL-1β, ( D ) <t>IL-18,</t> (E) TNF-α, and ( F ) IL-10 were quantified after exposure to 125 and 250 µg/mL nebulized LPS solution for 24 hours. Data are presented as mean ± SEM from three independent experiments. Statistical significance was determined using one-way ANOVA (*p< 0.5, **p < 0.01).
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    Cytokine secretion profile of CALU-3/dTHP-1 coculture following exposure to nebulized LPS. ( A ) IL-6, ( B ) IL-8, ( C ) IL-1β, ( D ) IL-18, (E) TNF-α, and ( F ) IL-10 were quantified after exposure to 125 and 250 µg/mL nebulized LPS solution for 24 hours. Data are presented as mean ± SEM from three independent experiments. Statistical significance was determined using one-way ANOVA (*p< 0.5, **p < 0.01).

    Journal: bioRxiv

    Article Title: Anti-oxidant and anti-inflammatory Effects of Aerosolised microalgal-derived extracellular vesicles in Bronchial Epithelial–Macrophage Co-cultures at the Air-Liquid Interface

    doi: 10.64898/2026.03.19.712886

    Figure Lengend Snippet: Cytokine secretion profile of CALU-3/dTHP-1 coculture following exposure to nebulized LPS. ( A ) IL-6, ( B ) IL-8, ( C ) IL-1β, ( D ) IL-18, (E) TNF-α, and ( F ) IL-10 were quantified after exposure to 125 and 250 µg/mL nebulized LPS solution for 24 hours. Data are presented as mean ± SEM from three independent experiments. Statistical significance was determined using one-way ANOVA (*p< 0.5, **p < 0.01).

    Article Snippet: The levels of the pro-inflammatory cytokine IL-8 and IL-18 were measured using the Human IL-8/CXCL8 DuoSet ELISA Kit (R&D Systems, DY208) and Human Total IL-18 DuoSet ELISA (R&D Systems, DY318) following the manufacturer’s recommendations.

    Techniques: